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20mM Glacial Acetic Acid and 1mM Na2EDTA
SIH Amino acids drop-out mixtures are used in combination with SIH Minimal medium w/o Amino acids or SIH Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium
Lysine and Methionine
Tryptone 10 Yeast extract 5 Final pH 7
Terrific Broth Size:6 x 1kg 20mM Glacial Acetic Acid andTerrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E. coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium. Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid. Tryptone and Yeast are present in the medium in elevated concentrations